Research Article

Protective Mechanism of Tong Fu Xie Fei on Lung Barrier Function in Acute Lung Injury Mouse Model

Xi Zhong, Wen-Hong Peng, Si-Yue Wang, Gang Wang, Xia Feng^✉

Department of Respiratory Medicine, Chongqing Hospital of Traditional Chinese Medicine, Chongqing 400021, China

✉, Correspondence
Xia Feng and Xi Zhong, Department of Respiratory Medicine, Chongqing Hospital of Traditional Chinese Medicine, Chongqing 400021, China. Email: [email protected]. Telephone number: 86-13608310261. 
Received: June 12, 2019; Accepted: August 24, 2019; Published online: October 16, 2019
Cite this paper: Xi Zhong, Wen-Hong Peng, Si-Yue Wang, Gang Wang, Xia Feng. (2020) Protective Mechanism of Tong Fu Xie Feng on Lung Barrier Function in Acute Lung Injury Mouse Model. Global Journal of Medicine, 1(1):22-29. https://naturescholars.com/gjm.010104. https://doi.org/10.46633/gjm.010104.
Copyright © 2020 by Scholars Publishing, LLC. 

Objective: To explore the protective mechanism of Tong Fu Xie Fei granule on lung barrier function in acute lung injury（ALI）of mice. Methods: Mice were randomly assigned to the following four groups, the control group, the model group, the treatment group and the SP group. Each group comprised of 15 mice. Lipopolysaccharide was injected intraperitoneally to induce ALI. Survival rates were recorded for 7 days after modeling. Expression of TNF-α and IL-6 in lung tissues and bronchoalveolar lavage fluid were measured using ELISA. mRNA and protein levels of ZO-1, VE-Cadherin and MLCK were ascertained by qRT-PCR or Western blotting. Optical microscopy was used to examine lung tissue for pathological changes. Results: Compared with the control group, there was obvious inflammatory response in the model group and SP group, the treatment group showed moderate inflammatory response and ameliorative lung barrier function than model group and SP group. Significant decreases in the expression levels of ZO-1 and VE-Cadherin, but increases in levels of MLCK and MLC were observed in model and excited group comparing with the control group. The expression levels of ZO-1 and VE-Cadherin were increased, but levels of MLCK and MLC were decreased significantly in the treatment group comparing with the model group and excited group. Conclusions: Tong Fu Xie Fei can reduce acute lung injury through effectively alleviating pulmonary inflammation and the activity of MLCK pathway, and enhancing the protective function of lung barrier.

Key words: Tongfuxiefei Formula; Acute Lung Injury (ALI); Endothelial barrier function; Myosin Light Chain Kinase (MLCK).