Research Article
The Effect of Terminalia Chebula on Metabolism of Radix Aconiti Kusnezoffii
Lulu Zhao✉, Shengnan Wang
Department of Pharmacology, College of Pharmacy, Inner Mongolian Medical University, Jinshan Development Zone, Hohhot, Inner Mongolia Autonomous Region, China. ✉ Correspondence Lulu Zhao, Department of Pharmacology, College of Pharmacy, Inner Mongolian Medical University, Jinshan Development Zone, Hohhot, Inner Mongolia Autonomous Region, 010110, China. Email: [email protected]. Telephone number: 86-471 6653 141. Received: January 5, 2020; Accepted: March 29, 2020; Published online: June 7, 2020. Cite this paper: Lulu Zhao, Shengnan Wang (2020) The Effect of Terminalia Chebula on Metabolism of Radix Aconiti Kusnezoffii. Global Journal of Life Sciences, 1(1): 24-33. https://naturescholars.com/gjls.010105. https://doi.org/10.46633/gjls.010105. Copyright© 2020 by Scholars Publishing, LLC.
Abstract
Objective At present, the domestic and foreign research on Terminalia chebula (HZ) and Radix Aconiti Kusnezoffii (CW) is mainly in the toxicity, but how HZ affects the metabolism of CW is not very clear. Therefore, this paper will focus on the study of whether the tannic acid in HZ has an effect on the metabolism of three kinds of double ester aconitine in CW. Methods CW is mainly composed of three components, namely aconitine (AC), mesaconine (MA) and hypaaconitine (HA). Metabolism of AC, MA and HA were studied in vitro in rat liver microsomes. Samples were taken at different time points. The residual concentration of three aconitines at different time points (0, 30, 60, 90, 120, 150, 180 min) was obtained. The metabolic rate was non-linear regression to the substrate concentration, thus, the curve of reaction speed to the substrate concentration was obtained. Finally, the metabolic parameters the maximum reaction rates (Vmax), the micron constants (Km) and the internal removal rates (CLint) of three aconitines were obtained. At the same time, HZ and CW were coincubated to determine whether HZ had any effect on the metabolism of CW. Results Vmax of AC, MA and HA are 387.59 ng·min－1·mg protein－1, 337.83 ng·min－1·mg protein－1 and 450 ng·min－1·mg protein－1; Km are 1124 ng·L－1, 962.8 ng·L－1 and 1278 ng·L－1. CLint are 0.34 μL·min－1·mg protein－1, 0.35 μL·min－1·mg protein－1 and 0.352 μL·min－1·mg protein－1，respectively. Conclusion HZ has a small inhibitory effect on the metabolism of CW.
Key word: Radix Aconiti Agrestis; Terminalia Chebula Retz; Compatibility ratio; Detoxification; Metabolism.