Research Article
Role of Nrf2 Signaling Pathway: Sulforaphane Enhances NGF-Induced Neurite Outgrowth in PC12 Cells
Rui Tang1,*, Sha Feng2,* Qianqian Cao3, and Ji-chun Zhang3,✉
1. School of Medicine, Xian Medicine College, Xian 710000, China. 2. Faculty of Medical Science, Jinan University, Guangzhou 510632, China. 3. Department of Physiology, School of Medicine, Jinan University, Guangzhou 510632, China.
* These authors contributed equally. ✉ Correspondence Ji-chun Zhang, Department of Physiology, School of Medicine, Jinan University, Guangzhou 510632, China. Email: [email protected]. Received: November 6, 2019; Accepted: December 18, 2020; Published online: December 28, 2020. Cite this paper: Rui Tang, Sha Feng, Qianqian Cao, and Ji-chun Zhang. (2020) Role of Nrf2 Signaling Pathway in Sulforaphane Enhances NGF-Induces Neurite Outgrowth in PC12 Cells. Global Journal of Neuroscience, 1(1):35-42. https://naturescholars.com/gjn.010106. https://doi.org/10.46633/gjn.010106. Copyright © 2020 by Scholars Publishing, LLC.
Abstract
Sulforaphane (SFN) is an organosulfur compound, which is found in cruciferous vegetables, such as broccoli Brussels sprouts, and cabbage. Accumulating evidence suggested that the mechanism for the antipsychotic drugs were promoting neurite outgrowth. Our recent study found that SFN showed antipsychotic effects. Therefore, it is interesting to investigate SFN enhanced NGF-induced neurite outgrowth in PC12 cells via Nrf2 signaling pathway. First, we found that SFN significantly enhanced NGF-induced neurite outgrowth in PC12 cells via the activation of Nrf2. Second, inositol 1,4,5-triphosphate (IP3) receptors inhibitors (Xestospongin C and 2-aminoethoxydiphenyl borate (2-APB)) significantly blocked SFN enhanced NGF-induced neurite outgrowth in PC12 cells. The results suggested that SFN enhanced NGF-induced neurite outgrowth in PC12 cells via Nrf2 signaling pathway, and subsequently via IP3 receptor.
Key words: Sulforaphane; NF-E2-related factor-2 (Nrf2); inositol 1,4,5-triphosphate (IP3); Neurite Outgrowth.